A case with clinically compatible signs and symptoms and at least two of the following hemorrhagic manifestations1:
- hemorrhagic or purpuric rash
- epistaxis
- hematemesis
- hemoptysis
- blood in stools
- other hemorrhagic symptom and no known predisposing host factors for hemorrhagic manifestations
AND
Laboratory confirmation of infection by one of the following methods:
- Isolation of dengue virus from an appropriate clinical specimen (blood, serum, tissue, urine specimens, or throat secretions)
- Detection of dengue virus nucleic acid by reverse-transcriptase PCR from blood, serum, or tissue
- Demonstration of dengue virus antigen in tissue (skin, liver, or spleen) by immunohistochemical or immunofluorescent techniques
- Demonstration of a significant (e.g. fourfold or greater rise) change in dengue virus neutralizing antibody titres in paired acute and convalescent sera by EIA, immunofluorescent assay, or Western Blot
- Demonstration of seroconversion of IgG or IgM antibodies by EIA, immunofluorescent assay, or Western Blot
- Demonstration of dengue virus antigen in blood, serum, or tissue by enzyme immunoassay (EIA ELISA)
A case with clinically compatible signs and symptoms and at least two of the following hemorrhagic manifestations1:
- hemorrhagic or purpuric rash
- epistaxis
- hematemesis
- hemoptysis
- blood in stools
- other hemorrhagic symptom and no known predisposing host factors for hemorrhagic manifestations
AND
Demonstration of a single elevated antibody titre by EIA, immunofluorescent assay or Western Blot
Notes:
- Only cases with hemorrhagic presentations are reportable.
